Comparison of Hemoglobin Levels by Copper Sulphate Method and Automated Hematology Analyzer in Blood Donors


  • Muhammad Ansar Department of Allied Health Sciences, Rawalpindi Medical University, Rawalpindi, Pakistan
  • Muhammad Saeed Department of Pathology, Tehsil Headquarters Hospital, Murree, Pakistan
  • Kanwal Shehzadi
  • Hira Ali
  • Muhammad Saifur Rehman Department of Biological Sciences, International Islamic University, Islamabad, Pakistan


Hemoglobin, Copper Sulphate, Blood Donors


Objective: To contrast the Hb results obtained using CuSO4 method and automated analyzer and to investigate how donor deferral is affected by the CuSO4 method's inaccuracy. Methodology: The study was conducted in Blood bank of Holy Family Hospital, Benazir Bhutto Hospital, District Headquarters Hospital and Hematology Laboratory, Pathology Department of Holy Family Hospital, Rawalpindi.

Capillary and venous blood sample were tested by using Hb screening appropriate standardized CuSO4 test (1.053) alongside the automated hematology analyzer, which was regarded as the industry standard reference technique.

Results: Results of copper sulphate were interpreted as Pass or Fail at Hb cut off of ≥12.5 g/dl. By this method, a total of 20 (16.6%) blood donors were deferred, out of which 15 were male and 5 were female. The percentage of deferrals was greater in women. (62.5%) in contrast to men. (13.3%) By Automated Hematology Analyzer (Sysmex-KX21) method, only 15 blood donors were deferred, out of which 11(10%) were male and 4 (50%) were female. The sensitivity of the CuSO4 was (95.24%), specificity (100%), PPV (100%), NPV (75%) and the sensitivity of automated hematology analyzer was (100%), specificity (100%), PPV (100%), NPV (100%).

Conclusion: The accuracy of CuSO4 was (95.83%) and the accuracy of the automated hematology analyzer is (100%). The inaccuracy of CuSO4 was 4.17%.






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